The lab says fixation will lead to the dyes to leak with the cells. I feel the idea is the fact fixation may hurt cell membranes. I did look for a paper which describes fixation working with methanol or acetic acid, immediately after staining having a DCFDA spinoff. DCFDA may https://elliottdlprs.blog-eye.com/28087294/manual-article-review-is-required-for-this-article